pmd2 g lenti helper plasmid gift (Addgene inc)
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pmd2 g lenti helper plasmid gift
Pmd2 G Lenti Helper Plasmid Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd2+g+lenti+helper+plasmid+gift/pMD2%2EG+(Plasmid+%2312259)/pm34739875-237-207-218
Average 98 stars, based on 14643 article reviews
Pmd2 G Lenti Helper Plasmid Gift, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd2+g+lenti+helper+plasmid+gift/pMD2%2EG+(Plasmid+%2312259)/pm34739875-237-207-218
Average 98 stars, based on 14643 article reviews
pmd2 g lenti helper plasmid gift - by Bioz Stars,
2026-09
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Virus:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Recombinant:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Article Title: Substratum stiffness regulates Erk signaling dynamics through receptor-level control Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMEM/F12 (3:1) without calcium Life Technologies Cat # 90-5010 Sodium bicarbonate Sigma Aldrich Cat # S5761 Transferrin Sigma Aldrich Cat # T2252 T3 (3,3’,5-triiodo-L-thyronine) Sigma Aldrich Cat # T2877 EGF, Alexa Fluor 488-conjugated Invitrogen Cat # E13345 Aminopropyltrimethoxysilane Sigma Aldrich Cat # 281778 Glutaraldehyde Sigma Aldrich Cat # 340855 40% acrylamide solution Bio-Rad Cat # 1610140 2% bis-acrylamide solution Bio-Rad Cat # 161-0142 Sulfo-SANPAH Thermo Fisher Scientific Cat # 22589 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Sigma Aldrich Cat # T9281 Ammonium persulfate (APS) Sigma Aldrich Cat # A3678 Fugene HD Promega Cat # E2311 Verso cDNA synthesis kit Thermo Fisher Scientific Cat # AB1453A iTaq Universal SYBR Green Supermix Bio-Rad Cat # 1725120 Experimental models: Cell lines MCF10A human mammary epithelial cells, clone 5E (Janes et al., 2010) RRID: CVCL_0598 ErkKTR-iRFP-2A-H2B-tRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP-2A-H2B-tRFP::BFP-SSPBSOScat-2A-PuroR-2A-iLID-CAAX (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::Myr-FusionRed-Cry2DropEGFR (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::TetON-shYAP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::YFP-YAP5SA (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::EGFR-FusionRed (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::H2B-RFP (CD-1 mouse primary keratinocytes) This paper N/A Lenti-X HEK 293T cells ClonTech Laboratories Cat # 632180 Oligonucleotides Human EGFR forward qRT-PCR primer: 5’ – CGTGGCAAGTCCC CCAGTGA – 3’ (Guturi et al., 2012) N/A Human EGFR reverse qRT-PCR primer: 5’ – GCAGACCAGGCAG TCGCTCTC– 3’ (Guturi et al., 2012) N/A Human 18S rRNA forward qRT-PCR primer: 5’ – CGGCGACGACCCAT TCGAAC – 3’ (Rabie et al., 2021) N/A Human 18S rRNA reverse qRT-PCR primer: 5’ – GAATCGAACCCTGAT TCCCCGTC – 3’ (Rabie et al., 2021) N/A (Continued on next page) Cell Reports 37, 110181, December 28, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR ErkKTR-iRFP-2A-H2B-tRFP (Goglia et al., 2020) N/A pHR BFP-SSPB-SOScat-2A-PuroR2A-iLID-CAAX (Goglia et al., 2020) N/A pHR ErkKTR-iRFP (Dine et al., 2018) Addgene # 111510 pHR EGFR-FusionRed This paper Addgene # 179263 pHR Myr-FusionRed-Cry2Drop-EGFR This paper Addgene # 179262 pCMV-dR8.91 lentivirus packaging plasmid Gift from Prof. Didier Trono, EPFL Addgene #12263 Polymerase Chain Reaction:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Cloning:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. DNA Extraction:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Plasmid Preparation:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Article Title: Substratum stiffness regulates Erk signaling dynamics through receptor-level control Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMEM/F12 (3:1) without calcium Life Technologies Cat # 90-5010 Sodium bicarbonate Sigma Aldrich Cat # S5761 Transferrin Sigma Aldrich Cat # T2252 T3 (3,3’,5-triiodo-L-thyronine) Sigma Aldrich Cat # T2877 EGF, Alexa Fluor 488-conjugated Invitrogen Cat # E13345 Aminopropyltrimethoxysilane Sigma Aldrich Cat # 281778 Glutaraldehyde Sigma Aldrich Cat # 340855 40% acrylamide solution Bio-Rad Cat # 1610140 2% bis-acrylamide solution Bio-Rad Cat # 161-0142 Sulfo-SANPAH Thermo Fisher Scientific Cat # 22589 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Sigma Aldrich Cat # T9281 Ammonium persulfate (APS) Sigma Aldrich Cat # A3678 Fugene HD Promega Cat # E2311 Verso cDNA synthesis kit Thermo Fisher Scientific Cat # AB1453A iTaq Universal SYBR Green Supermix Bio-Rad Cat # 1725120 Experimental models: Cell lines MCF10A human mammary epithelial cells, clone 5E (Janes et al., 2010) RRID: CVCL_0598 ErkKTR-iRFP-2A-H2B-tRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP-2A-H2B-tRFP::BFP-SSPBSOScat-2A-PuroR-2A-iLID-CAAX (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::Myr-FusionRed-Cry2DropEGFR (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::TetON-shYAP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::YFP-YAP5SA (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::EGFR-FusionRed (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::H2B-RFP (CD-1 mouse primary keratinocytes) This paper N/A Lenti-X HEK 293T cells ClonTech Laboratories Cat # 632180 Oligonucleotides Human EGFR forward qRT-PCR primer: 5’ – CGTGGCAAGTCCC CCAGTGA – 3’ (Guturi et al., 2012) N/A Human EGFR reverse qRT-PCR primer: 5’ – GCAGACCAGGCAG TCGCTCTC– 3’ (Guturi et al., 2012) N/A Human 18S rRNA forward qRT-PCR primer: 5’ – CGGCGACGACCCAT TCGAAC – 3’ (Rabie et al., 2021) N/A Human 18S rRNA reverse qRT-PCR primer: 5’ – GAATCGAACCCTGAT TCCCCGTC – 3’ (Rabie et al., 2021) N/A (Continued on next page) Cell Reports 37, 110181, December 28, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR ErkKTR-iRFP-2A-H2B-tRFP (Goglia et al., 2020) N/A pHR BFP-SSPB-SOScat-2A-PuroR2A-iLID-CAAX (Goglia et al., 2020) N/A pHR ErkKTR-iRFP (Dine et al., 2018) Addgene # 111510 pHR EGFR-FusionRed This paper Addgene # 179263 pHR Myr-FusionRed-Cry2Drop-EGFR This paper Addgene # 179262 pCMV-dR8.91 lentivirus packaging plasmid Gift from Prof. Didier Trono, EPFL Addgene #12263 Software:Article Title: A synthetic gene circuit for imaging-free detection of signaling pulses. Article Snippet: In brief Signaling pathways often exhibit discrete pulses of activity whose sources and functions remain poorly understood.. Ravindran et al. discovered a network motif that selectively responds to signaling pulses, rather than constant high or low signaling states.. They implemented this motif in a synthetic gene circuit for the Erk signaling pathway and characterized pulse detection in response to spontaneous and externally applied dynamics. Article Title: Substratum stiffness regulates Erk signaling dynamics through receptor-level control Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMEM/F12 (3:1) without calcium Life Technologies Cat # 90-5010 Sodium bicarbonate Sigma Aldrich Cat # S5761 Transferrin Sigma Aldrich Cat # T2252 T3 (3,3’,5-triiodo-L-thyronine) Sigma Aldrich Cat # T2877 EGF, Alexa Fluor 488-conjugated Invitrogen Cat # E13345 Aminopropyltrimethoxysilane Sigma Aldrich Cat # 281778 Glutaraldehyde Sigma Aldrich Cat # 340855 40% acrylamide solution Bio-Rad Cat # 1610140 2% bis-acrylamide solution Bio-Rad Cat # 161-0142 Sulfo-SANPAH Thermo Fisher Scientific Cat # 22589 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Sigma Aldrich Cat # T9281 Ammonium persulfate (APS) Sigma Aldrich Cat # A3678 Fugene HD Promega Cat # E2311 Verso cDNA synthesis kit Thermo Fisher Scientific Cat # AB1453A iTaq Universal SYBR Green Supermix Bio-Rad Cat # 1725120 Experimental models: Cell lines MCF10A human mammary epithelial cells, clone 5E (Janes et al., 2010) RRID: CVCL_0598 ErkKTR-iRFP-2A-H2B-tRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP-2A-H2B-tRFP::BFP-SSPBSOScat-2A-PuroR-2A-iLID-CAAX (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::Myr-FusionRed-Cry2DropEGFR (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::TetON-shYAP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::YFP-YAP5SA (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::EGFR-FusionRed (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::H2B-RFP (CD-1 mouse primary keratinocytes) This paper N/A Lenti-X HEK 293T cells ClonTech Laboratories Cat # 632180 Oligonucleotides Human EGFR forward qRT-PCR primer: 5’ – CGTGGCAAGTCCC CCAGTGA – 3’ (Guturi et al., 2012) N/A Human EGFR reverse qRT-PCR primer: 5’ – GCAGACCAGGCAG TCGCTCTC– 3’ (Guturi et al., 2012) N/A Human 18S rRNA forward qRT-PCR primer: 5’ – CGGCGACGACCCAT TCGAAC – 3’ (Rabie et al., 2021) N/A Human 18S rRNA reverse qRT-PCR primer: 5’ – GAATCGAACCCTGAT TCCCCGTC – 3’ (Rabie et al., 2021) N/A (Continued on next page) Cell Reports 37, 110181, December 28, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR ErkKTR-iRFP-2A-H2B-tRFP (Goglia et al., 2020) N/A pHR BFP-SSPB-SOScat-2A-PuroR2A-iLID-CAAX (Goglia et al., 2020) N/A pHR ErkKTR-iRFP (Dine et al., 2018) Addgene # 111510 pHR EGFR-FusionRed This paper Addgene # 179263 pHR Myr-FusionRed-Cry2Drop-EGFR This paper Addgene # 179262 pCMV-dR8.91 lentivirus packaging plasmid Gift from Prof. Didier Trono, EPFL Addgene #12263 other:Article Title: Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHR-iLID-Only Zap70K362E This paper N/A pHR-iLID-Drop Zap70K369R This paper N/A Clone Assay:Article Title: Substratum stiffness regulates Erk signaling dynamics through receptor-level control Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMEM/F12 (3:1) without calcium Life Technologies Cat # 90-5010 Sodium bicarbonate Sigma Aldrich Cat # S5761 Transferrin Sigma Aldrich Cat # T2252 T3 (3,3’,5-triiodo-L-thyronine) Sigma Aldrich Cat # T2877 EGF, Alexa Fluor 488-conjugated Invitrogen Cat # E13345 Aminopropyltrimethoxysilane Sigma Aldrich Cat # 281778 Glutaraldehyde Sigma Aldrich Cat # 340855 40% acrylamide solution Bio-Rad Cat # 1610140 2% bis-acrylamide solution Bio-Rad Cat # 161-0142 Sulfo-SANPAH Thermo Fisher Scientific Cat # 22589 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Sigma Aldrich Cat # T9281 Ammonium persulfate (APS) Sigma Aldrich Cat # A3678 Fugene HD Promega Cat # E2311 Verso cDNA synthesis kit Thermo Fisher Scientific Cat # AB1453A iTaq Universal SYBR Green Supermix Bio-Rad Cat # 1725120 Experimental models: Cell lines MCF10A human mammary epithelial cells, clone 5E (Janes et al., 2010) RRID: CVCL_0598 ErkKTR-iRFP-2A-H2B-tRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP-2A-H2B-tRFP::BFP-SSPBSOScat-2A-PuroR-2A-iLID-CAAX (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::Myr-FusionRed-Cry2DropEGFR (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::TetON-shYAP (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::YFP-YAP5SA (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::EGFR-FusionRed (MCF10A, clone 5E) This paper N/A ErkKTR-iRFP::H2B-RFP (CD-1 mouse primary keratinocytes) This paper N/A Lenti-X HEK 293T cells ClonTech Laboratories Cat # 632180 Oligonucleotides Human EGFR forward qRT-PCR primer: 5’ – CGTGGCAAGTCCC CCAGTGA – 3’ (Guturi et al., 2012) N/A Human EGFR reverse qRT-PCR primer: 5’ – GCAGACCAGGCAG TCGCTCTC– 3’ (Guturi et al., 2012) N/A Human 18S rRNA forward qRT-PCR primer: 5’ – CGGCGACGACCCAT TCGAAC – 3’ (Rabie et al., 2021) N/A Human 18S rRNA reverse qRT-PCR primer: 5’ – GAATCGAACCCTGAT TCCCCGTC – 3’ (Rabie et al., 2021) N/A (Continued on next page) Cell Reports 37, 110181, December 28, 2021 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR ErkKTR-iRFP-2A-H2B-tRFP (Goglia et al., 2020) N/A pHR BFP-SSPB-SOScat-2A-PuroR2A-iLID-CAAX (Goglia et al., 2020) N/A pHR ErkKTR-iRFP (Dine et al., 2018) Addgene # 111510 pHR EGFR-FusionRed This paper Addgene # 179263 pHR Myr-FusionRed-Cry2Drop-EGFR This paper Addgene # 179262 pCMV-dR8.91 lentivirus packaging plasmid Gift from Prof. Didier Trono, EPFL Addgene #12263 |